Effects of pituitary adenylate cyclase activating polypeptide on CD4+/CD8+ T cell levels after traumatic brain injury in a rat model

doi:10.5847/wjem.j.issn.1920-8642.2012.04.010

Abstract

Abstract:

BACKGROUND: The effect of pituitary adenylate cyclase activating polypeptide (PACAP) during traumatic brain injury (TBI) and whether it can modulate secondary injury has not been reported previously. The present study evaluated the potential protective effects of ventricular infusion of PACAP in a rat model of TBI.

METHODS: Male Sprague Dawley rats were randomly divided into 3 treatment groups (n=6, each): sham-operated, vehicle (normal saline)+TBI, and PACAP+TBI. Normal saline or PACAP (1 μg/5 μL) was administered intracerebroventricularly 20 minutes before TBI. Right parietal cortical contusion was produced via a weight-dropping method. Brains were extracted 24 hours after trauma. Histological changes in brains were examined by HE staining. The numbers of CD4⁺ and CD8⁺ T cells in blood and the spleen were detected via flow cytometry.

RESULTS: In injured brain regions, edema, hemorrhage, inflammatory cell infiltration, and swollen and degenerated neurons were observed under a light microscope, and the neurons were disorderly arrayed in the hippocampi. Compared to the sham group, average CD4⁺ CD8^- lymphocyte counts in blood and the spleen were significantly decreased in rats that received TBI+vehicle, and CD4^- CD8⁺ were increased. In rats administered PACAP prior to TBI, damage was attenuated as evidenced by significantly increased CD4⁺, and decreased CD8⁺, T lymphocytes in blood and the spleen.

CONCLUSION: Pretreatment with PACAP may protect against TBI by influencing periphery T cellular immune function.

Key words: Traumatic brain injury, Pituitary adenylate cyclase activating polypeptide, CD4⁺ T lymphocyte, CD8⁺ T lymphocyte, Rat, Spleen, Blood, Flow cytometry

Rong Hua, Shan-shan Mao, Yong-mei Zhang, Fu-xing Chen, Zhong-hai Zhou, Jun-quan Liu. Effects of pituitary adenylate cyclase activating polypeptide on CD4⁺/CD8⁺ T cell levels after traumatic brain injury in a rat model[J]. World Journal of Emergency Medicine, 2012, 3(4): 294-298.

Figures/Tables 6

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Group	CD4⁺ T cells	CD8⁺ T cells
Sham	59.57±4.97	13.54±4.51
NS+TBI	39.20±2.44^*	24.62±6.27^*
PACAP+TBI	48.26±6.29^#	18.34±5.09

Groups	CD4⁺ T cells	CD8⁺ T cells
Sham	43.14±3.73	17.43±3.76
NS+ TBI	25.74±4.46^#	23.26±4.13^#
PACAP+ TBI	28.37±11.93^*	19.49±2.11

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