World Journal of Emergency Medicine ›› 2011, Vol. 2 ›› Issue (3): 216-221.doi: 10.5847/wjem.j.1920-8642.2011.03.011
• Original Article • Previous Articles Next Articles
Kai Yin, Sheng-chun Dang, Jian-xin Zhang()
Received:
2011-04-07
Accepted:
2011-08-22
Online:
2011-09-15
Published:
2011-09-15
Contact:
Jian-xin Zhang
E-mail:zhangjx@ujs.edu.cn
Kai Yin, Sheng-chun Dang, Jian-xin Zhang. Relationship between expression of triggering receptor-1 on myeloid cells in intestinal tissue and intestinal barrier dysfunction in severe acute pancreatitis[J]. World Journal of Emergency Medicine, 2011, 2(3): 216-221.
Add to citation manager EndNote|Ris|BibTeX
URL: http://wjem.com.cn//EN/10.5847/wjem.j.1920-8642.2011.03.011
Table 1
The primer sequences and amplified fragment length
Primer | PCR product (bp) | |
---|---|---|
TREM-1 | 5'-AAGTATGCCAGAAGCAGGAAGG-3' | 138 |
5'-GGTAGGGTCATCTTTCAGGGTGT-3' | ||
IL-1β | 5'-AATACCACTTGTTGGCTTA-3' | 130 |
5'-TGTGATGTTCCCATTAGAC-3' | ||
TNF-α | 5'-CGTCGTAGCAAACCACCAAG-3' | 426 |
5'-CACAGAGCAATGACTCCAAAG-3' | ||
β-actin | 5'-CCTGTACGCCAACACAGTGC-3' | 211 |
5'-ATACTCCTGCTTGCTGATCC-3' |
Table 5
Comparison of TREM-1,IL-1β and TNF-α mRNA in rat intestine at different time points between the two groups (mean±SD)
Group | TREM-1 | IL-1β | TNF-α |
---|---|---|---|
SO | 0.185±0.060 | 0.116±0.035 | 0.136±0.023 |
SAP 2 h | 0.330±0.028* | 0.457±0.068* | 0.547±0.080* |
SAP 6 h | 0.348±0.030* | 0.490±0.049* | 0.645±0.061** |
SAP 12 h | 0.427±0.043* | 0.598±0.054** | 0.689±0.050* |
SAP 24 h | 0.407±0.050* | 0.622±0.101** | 0.589±0.037* |
Figure 3.
Morphological changes in intestinal mucosa after induction of SAP (HE, original magnification × 100). A: Intestinal section with normal mucosa histopathologically graded as 0; B: Intestinal section with massive epithelial lifting histopathologically graded as 3; C: Intestinal mucosa with denuded villi histopathologically graded as 4; D: Intestinal mucosa with disintegration of the lamina propria graded as 5.
1 |
Nakajima T, Ueda T, Takeyama Y, Yasuda T, Shinzeki M, Sawa H, et al. Protective effects of vascular endothelial growth factor on intestinal epithelial apoptosis and bacterial translocation in experimental severe acute pancreatitis. Pancreas 2007; 34:410-416.
doi: 10.1097/mpa.0b013e3180335c64 pmid: 17446839 |
2 |
Dang SC, Zhang JX, Qu JG, Mao ZF, Wang XQ, Zhu B. Dynamic changes of IL-2/IL-10, sFas and expression of Fas in intestinal mucosa in rats with acute necrotizing pancreatitis. World J Gastroenterol 2008; 14:2246-2250.
doi: 10.3748/wjg.14.2246 pmid: 18407603 |
3 |
Bouchon A, Facchetti F, Weigand MA, Colonna M. TREM-1 amplifies inflammation and is a crucial mediator of septic shock. Nature 2001; 410:1103-1107.
doi: 10.1038/35074114 pmid: 11323674 |
4 |
Klesney-Tait J, Turnbull IR, Colonna M. The TREM receptor family and signal integration. Nat Immunol 2006; 7:1266-1273.
doi: 10.1038/ni1411 pmid: 17110943 |
5 |
Nathan C, Ding A. TREM-1: a new regulator of innate immunity in sepsis syndrome. Nat Med. 2001; 7:530-532.
doi: 10.1038/87846 pmid: 11329047 |
6 | Wang DY, Qin RY, Liu ZR, Gupta MK, Chang Q. Expression of TREM-1 mRNA in acute pancreatitis. World[J] Gastroenterol. 2004; 10:2744-2746. |
7 | Schenk M, Bouchon A, Seibold F, Mueller C. TREM-1-expressing intestinal macrophages crucially amplify chronic inflammation in experimental colitis and inflammatory bowel diseases. J Clin Invest 2007; 117:3097-106. |
8 |
Kamei K, Yasuda T, Ueda T, Qiang F, Takeyama Y, Shiozaki H. Role of triggering receptor expressed on myeloid cells-1 in experimental severe acute pancreatitis. J Hepatobiliary Pancreat Sci 2010; 17: 305-312. Epub 2009 Sep 29.
doi: 10.1007/s00534-009-0191-6 pmid: 19787284 |
9 |
Chiu CJ, McArdle AH, Brown R, Scott HJ, Gurd FN Intestinal mucosal lesion in low-flow states. I. A morphological, hemodynamic, and metabolic reappraisal. Arch Surg 1970; 101:478-483.
doi: 10.1001/archsurg.1970.01340280030009 pmid: 5457245 |
10 |
Park JJ, Cheon JH, Kim BY, Kim DH, Kim ES, Kim TI, et al. Correlation of serum-soluble triggering receptor expressed on myeloid cells-1 with clinical disease activity in inflammatory bowel disease. Dig Dis Sci 2008; 54:1525-1531.
doi: 10.1007/s10620-008-0514-5 pmid: 18975078 |
11 |
Tzivras M, Koussoulas V, Giamarellos-Bourboulis EJ, Tzivras D, Tsaganos T, Koutoukas P, et al. Role of soluble triggering receptor expressed on myeloid cells in inflammatory bowel disease. World J Gastroenterol 2006; 12:3416-3419.
doi: 10.3748/wjg.v12.i21.3416 pmid: 16733861 |
12 |
González-Roldán N, Ferat-Osorio E, Aduna-Vicente R, Wong-Baeza I, Esquivel-Callejas N, Astudillo-de la Vega H, et al. Expression of triggering receptor on myeloid cell 1 and histocompatibility complex molecules in sepsis and major abdominal surgery. World J Gastroenterol 2005; 11:7473-7479.
doi: 10.3748/wjg.v11.i47.7473 pmid: 16437719 |
13 |
Ferat-Osorio E, Wong-Baeza I, Esquivel-Callejas N, Figueroa-Figueroa S, Duarte-Rojo A, Guzmán-Valdivia-Gómez G, et al. Triggering receptor expressed on myeloid cells-1 expression on monocytes is associated with inflammation but not with infection in acute pancreatitis. Crit Care 2009; 13:R69.
doi: 10.1186/cc7876 pmid: 19442309 |
14 |
Morencos FC, de las Heras Castaño G, Martín Ramos L, López Arias MJ, Ledesma F, Pons Romero F. Small bowel bacterial overgrowth in patients with alcoholic cirrhosis. Dig Dis Sci 1995; 40:1252-1256.
pmid: 7781442 |
15 |
Kitanaka J, Kitanaka N, Tsujimura T, Terada N, Takemura M. Expression of diamine oxidase (histaminase) in guinea-pig tissues. Eur J Pharmacol 2002; 437:179-185.
doi: 10.1016/s0014-2999(02)01302-x pmid: 11890907 |
16 |
Peng X, Yan H, You Z, Wang P, Wang S. Effects of enteral supplementation with glutamine granules on intestinal mucosal barrier function in severe burned patients. Burns 2004; 30:135-139.
doi: 10.1016/j.burns.2003.09.032 pmid: 15019120 |
17 |
Zhang X-p, Zhang J, Song Q-l, Chen HQ. Mechanism of acute pancreatitis complicated with injury of intestinal mucosa barrier. J Zhejiang Univ Sci B 2007; 8:888-895.
doi: 10.1631/jzus.2007.B0888 pmid: 18257123 |
18 |
Pereda J, Sabater L, Cassinello N, Gómez-Cambronero L, Closa D, Folch-Puy E, et al. Effect of simultaneous inhibition of TNF-alpha production and xanthine oxidase in experimental acute pancreatitis: the role of mitogen activated protein kinases. Ann Surg 2004; 240:108-116.
pmid: 15213626 |
19 |
Strobel O, Wachter D, Werner J, Uhl W, Müller CA, Khalik M, et al. Effect of a pneumoperitoneum on systemic cytokine levels, bacterial translocation, and organ complications in a rat model of severe acute pancreatitis with infected necrosis. Surgical Endoscopy 2006; 20:1897-903.
doi: 10.1007/s00464-005-0417-x pmid: 17024542 |
20 |
Aggarwal BB. Signalling pathways of the TNF superfamily: a double-edged sword. Nat Rev Immunol 2003; 3:745-756.
doi: 10.1038/nri1184 pmid: 12949498 |
21 |
Granger J, Remick D. Acute pancreatitis: models, markers, and mediators. Shock 2005; 24(Suppl 1):45-51.
doi: 10.1097/01.shk.0000191413.94461.b0 |
22 |
Mole DJ, Taylor MA, McFerran NV, Diamond T. The isolated perfused liver response to a 'second hit' of portal endotoxin during severe acute pancreatitis. Pancreatology 2005; 5:475-485.
doi: 10.1159/000086614 pmid: 15985775 |
23 |
Gong JP, Wu CX, Liu CA, Li SW, Shi YJ, Yang K, et al. Intestinal damage mediated by Kupffer cells in rats with endotoxemia. World J Gastroenterol 2002; 8:923-927.
doi: 10.3748/wjg.v8.i5.923 pmid: 12378643 |
24 |
Leveau P, Wang X, Sun Z, Börjesson A, Andersson E, Andersson R. Severity of pancreatitis-associated gut barrier dysfunction is reduced following treatment with the PAF inhibitor lexipafant. Biochemical Pharmacology 2005; 69:1325-1331.
doi: 10.1016/j.bcp.2005.01.023 |
[1] | Xiang-wang Zhao, Lei Yan, Dan Xu, Yu-hui Cui, Chun-hui Yang, Yan-jun Zhou, Jian-guo Tang. Enterogenous infection of Candida albicans in immunocompromised rats under severe acute pancreatitis [J]. World Journal of Emergency Medicine, 2016, 7(4): 294-299. |
[2] | Ming Wei, Yan-jie Gong, Ling Tu, Jia Li, Ying-hong Liang, Yi-hua Zhang. Expression of phosphatidylinositol-3 kinase and effects of inhibitor Wortmannin on expression of tumor necrosis factor-α in severe acute pancreatitis associated with acute lung injury [J]. World Journal of Emergency Medicine, 2015, 6(4): 299-304. |
[3] | Liang-shan Peng, Juan Li, Gao-sheng Zhou, Lie-hua Deng, Hua-guo Yao. Relationships between genetic polymorphisms of triggering receptor expressed on myeloid cells-1 and septic shock in a Chinese Han population [J]. World Journal of Emergency Medicine, 2015, 6(2): 123-130. |
[4] | Hui-ming Zhu, Shao-qing Guo, Xiu-min Liao, Li Zhang, Li Cai. Embryonic natural orifice transluminal endoscopic surgery in the treatment of severe acute pancreatitis complicated by abdominal compartment syndrome [J]. World Journal of Emergency Medicine, 2015, 6(1): 23-28. |
[5] | Ying-zhen Wang, Shi-wen Wang, You-cheng Zhang, Zhi-jiang Sun. Protective effect of exogenous IGF-I on the intestinal mucosal barrier in rats with severe acute pancreatitis [J]. World Journal of Emergency Medicine, 2012, 3(3): 213-220. |
[6] | Xiao-yan Li, Xiao-bo Wang, Xiu-feng Liu, Shu-gui Li. Prevalence and risk factors of organ failure in patients with severe acute pancreatitis [J]. World Journal of Emergency Medicine, 2010, 1(3): 201-204. |
[7] | Xin-bo Ai, Xiao-ping Qian, Wen-sheng Pan, Jun Xu, Liang-qing Wu, Wan-jun Zhang, An Wang. Ultrasound-guided percutaneous catheter drainage in early treatment of severe acute pancreatitis [J]. World Journal of Emergency Medicine, 2010, 1(1): 45-48. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||