Effects of hyperbaric oxygen on intestinal mucosa apoptosis caused by ischemia-reperfusion injury in rats

doi:10.5847/wjem.j.issn.1920-8642.2012.02.010

Abstract

Abstract:

BACKGROUND: Hyperbaric oxygen (HBO) is an effective adjuvant therapy for ischemia- reperfusion (I/R) injury of the brain, small intestine and testis in addition to crushing injury. Studies have shown that HBO increases the activity of villi of the ileum 30 minutes after I/R injury. The present study aimed to observe the effect of HBO on apoptosis of epithelial cells in the small intestine during different periods of I/R and to elucidate the potential mechanisms.

METHODS: Rats were subjected to 60-minute ischemia by clamping the superior mesenteric artery and 60-minute reperfusion by removal of clamping. The rats were randomly divided into four groups: I/R group, HBO precondition or HBO treatment before ischemia (HBO-P), HBO treatment during ischemia period (HBO-I), and HBO treatment during reperfusion (HBO-R). After 60-minute reperfusion, samples of the small intestine were prepared to measure the level of ATP by using the colorimetric method and immunochemical expression of caspase-3. The levels of TNF-α in intestinal tissue were measured using the enzyme-linked immunosorbent assay method (Elisa).

RESULTS: TNF-α levels were significantly lower in the HBO-I group than in the HBO-P (P<0.05), HBO-R and I/R groups; there was no significant difference between the HBO-R and I/R groups (P>0.05). The expression of caspas-3 was significantly lower in the HBO-I group than in the HBO-P group (P<0.05); it was also significantly lower in the HBO-P group than in the I/R and HBO-R groups (P<0.05). ATP level was significantly lower in the HBO-I group than in the HBO-P group (P<0.05), and also it was significantly lower in the HBO-P group than in the I/R and HBO-R groups (P<0.05).

CONCLUSIONS: There is an association between HBO, small intestinal I/R injury, and mucosa apoptosis. HBO maintains ATP and aerobic metabolism, inhibites TNF-α production, and thus prevents intestinal mucosa from apoptosis. Best results can be obtained when HBO is administered to patients in the period of ischemia, and no side effects are produced when HBO is given during the period of reperfusion.

Key words: Hyperbaric oxygen, Ischemia-reperfusion injury, Apoptosis

Shi-hui Zhou, Yan-fei Sun, Gang Wang. Effects of hyperbaric oxygen on intestinal mucosa apoptosis caused by ischemia-reperfusion injury in rats[J]. World Journal of Emergency Medicine, 2012, 3(2): 135-140.

Figures/Tables 2

References 32

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Parameters	I/R group	HBO-R group	HBO-P group	HBO-I group	F value
TNF-α (pg/g)	1606.9±103.25^*	1536.2±136.17^*	1252.8±109.02^▲*	1021.9±91.29^▲	58.709
ATP enzyme (U/mg)	10.86±1.04^*	11.42±0.84^*	13.88±1.05^▲	16.97±1.45^▲	62.224
Optical density	22.059±1.05^*	21.320±0.89^*	15.581±0.72^▲	14.186±0.61^▲	226.954

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