World Journal of Emergency Medicine ›› 2015, Vol. 6 ›› Issue (3): 201-206.doi: 10.5847/wjem.j.1920-8642.2015.03.007
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Şükrü Gürbüz1(), Mustafa Yıldız2, Murat Kara3, Kürşat Kargün4, Mehtap Gürger2, Metin Ateşçelik2, Ömer Doğan Alataş5
Received:
2015-02-03
Accepted:
2015-07-11
Online:
2015-09-15
Published:
2015-09-15
Contact:
Şükrü Gürbüz
E-mail:sukrugurbuz@gmail.com
Şükrü Gürbüz, Mustafa Yıldız, Murat Kara, Kürşat Kargün, Mehtap Gürger, Metin Ateşçelik, Ömer Doğan Alataş. Paraoxonase-1 gene in patients with chronic obstructive pulmonary disease investigation Q192R and L55M polymorphisms[J]. World Journal of Emergency Medicine, 2015, 6(3): 201-206.
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URL: http://wjem.com.cn//EN/10.5847/wjem.j.1920-8642.2015.03.007
Figure 1.
Image of PCR products-related to PON1 192 polymorphism on agarose gel electrophoresis. Alw1 enzyme was used for cutting PCR products. Subjects 1 and 3 have QQ genotype, while subjects 7, 11, 15, 19, 27 have QR and subject 40 has RR genotype. Q allele at 99 bp; R allele at 69 and 30 bp. M: 50-1 000 bp size marker.
Figure 2.
Image of PCR products-related to PON1 55 polymorphism on agarose gel electrophoresis. Hsp91II enzyme was used for cutting PCR products. Subjects 1 and 3 have LL genotype, while subjects 7, 11, 15, have LM and subjects 19, 27, 40 have MM genotype. L allele at 170 bp; M allele at 170 and 126 bp. M: 126 bp size marker.
Table 1
Primary sequences used for PON 192 and PON 55 gene regions
Primary sequences used for PON 192 gene region 5'-TAT TGT TGC TGT GGG ACC TGA G-3' |
5'-CAC GCT AAA CCC AAA TAC ATC TC-3' |
Primary sequences used for PON 55 gene region |
5'-GAA GAG TGA TGT ATA GCC CCA G-3' |
5'-TTT AAT CCA GAG CTA ATG AAA GCC-3' |
Table 6
Genotype and allele distributions of PON1 gene Q192R and L55M polymorphisms
Gene | Genotype Allele | Patients (n=51) | Controls (n=40) | χ2 | P |
---|---|---|---|---|---|
PON1 gene Q192R | 33 (0.647) | 32 (0.800) | 4.386 | 0.111 | |
QR | 14 (0.275) | 8 (0.200) | |||
RR | 4 (0.078) | 0 (0.000) | |||
Q | 80 (0.784) | 72 (0.900) | 4.358 | 0.036 | |
R | 22 (0.216) | 8 (0.100) | |||
PON1 gene L55M | LL | 30 (0.612) | 25 (0.595) | 0.950 | 0.621 |
LM | 18 (0.367) | 17 (0.405) | |||
MM | 1 (0.020) | 0 (0.000) | |||
L | 78 (0.796) | 67 (0.798) | 0.0008 | 0.977 | |
M | 20 (0.204) | 17 (0.202) |
Table 7
Association of risk factors with gene polymorphisms
COPD factors | Q192R | L55M | ||||
---|---|---|---|---|---|---|
QQ (n, %) | QR (n, %) | RR (n, %) | LL (n, %) | LM (n, %) | MM (n, %) | |
Family history | 8 (15.7) | 3 (5.9) | 1 (2.0) | 5 (10.2) | 8 (16.3) | 1 (2.0) |
Smoking | 24 (47.1) | 5 (9.8) | 3 (5.9) | 18 (36.7) | 12 (24.5) | 1 (2.0) |
Tbc | 2 (3.9) | 1 (2.0) | 0 (0) | 2 (4.1) | 1 (2.0) | 1 (2.0) |
Respiratory tract infections | 12 (23.5) | 8 (15.7) | 2 (3.9) | 14 (28.6) | 7 (14.3) | 0 (0) |
Socieconomic status | 5 (9.8) | 2 (3.9) | 1 (2.0) | 4 (8.2) | 4 (8.2) | 0 (0) |
Nutrition | 5 (9.8) | 1 (2.0) | 1 (2.0) | 4 (8.2) | 3 (6.1) | 0 (0) |
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