Figure 1.

Optimization of the conditions for the rapid quantitative method test using a spectrophotometer. A: absorption spectra of the different liquids (n=5); QC solutions (50, 200, and 500 μg/mL) and blank gastric juice were scanned at 250-450 nm with a spectrophotometer after mixing with the detection solution. The maximum absorption wavelengths of the liquids all appeared at 395 nm; QC solutions: quality control solutions; B-C: the absorbance at 395 nm varies with changes in the concentrations of NaOH (B) and Na2S2O4 (C) (n=5). The maximum numerical value was detected in 2.5% to 15.0% NaOH and 7.5% Na2S2O4; D: variations in absorbance at 395 nm versus time (n=5). The numerical values of the measurements in the first ten minutes were stable. The numerical values dropped by 31.1%, 66.2%, and 58.9% for the QC solutions of 50 μg/mL, 200 μg/mL, and 500 μg/mL, respectively, at 30 minutes.

Table 1

Precision and accuracy of QC solutions

Figure 2.

The trend of PQ concentration in the gastric juices and plasma samples of each group in different time points (H1: at 1 hour, H6: at 6 hours, H24: at 24 hours). A: comparison of the PQ concentrations in the gastric juices of each group; *P<0.01 for the difference between H1 and H6, #P<0.01 between H1 and H24, and &P<0.01 between H6 and H24; B: comparison of the PQ concentrations in the plasma of each group at three different time points; *P<0.01 for the difference between H1 and H6, &P<0.05 between H6 and H24.

Table 2

Comparisons of PQ concentration in GL eluate between each group (μg/mL)

Figure 3.

Comparison of the PQ clearance efficiencies between the first 10 L GL and the second 10 L GL. *P<0.01 using ANOVA for the difference in the PQ+H1 GL between the first 10 L GL and the second 10 L GL; **P<0.01 using ANOVA for the difference in the PQ+H6 GL between the first 10 L GL and the second 10 L GL.

Table 3

Vital signs parameters between each group

Table 4

Laboratory examination at H24 between each group