The relationship between platelet endothelial cell adhesion molecule-1 and paraquat-induced lung injury in rabbits

doi:10.5847/wjem.j.issn.1920-8642.2012.01.011

Abstract

Abstract:

BACKGROUND: Platelet endothelial cell adhesion molecule-1 (PECAM-1), also known as CD31, is mainly distributed in vascular endothelial cells. Studies have shown that PECAM-1 is a very significant indicator of angiogenesis, and has been used as an indicator for vascular endothelial cells. The present study aimed to explore the relationship between the expression of PECAM-1 and the degree of acute lung injury (ALI) and fibrosis in paraquat (PQ) induced lung injury in rabbits.

METHODS: Thirty-six adult New Zealand rabbits were randomly divided into three groups (12 rabbits in each group) according to PQ dosage: 8 mg/kg (group A), 16 mg/kg (group B), and 32 mg/kg (group C). After PQ infusion, the rabbits were monitored for 7 days and then euthanized. The lungs were removed for histological evaluation. Masson staining was used to determine the degree of lung fibrosis (LF), and semi-quantitative immune-histochemistry analysis to determine the expression of PECAM-1. Pearson's product-moment correlation analysis was performed to evaluate the relationship between the expression of PECAM-1 and the extent of lung injuries expressed by ALI score and degree of LF.

RESULTS: Rabbits in the three groups showed apparent poisoning. The rabbits survived longer in group A than in groups B and C (6.47±0.99 days vs. 6.09±1.04 days vs. 4.77±2.04 days) (P<0.05). ALI score was lower in group A than in groups B and C (8.33±1.03 vs. 9.83±1.17 vs. 11.50±1.38) (P<0.05), and there was statistically significant difference between group B and group C (P=0.03). LF was slighter in group A than in groups B and C (31.09%±2.05 % vs. 34.37%±1.62 % vs. 36.54%±0.44%) (P<0.05), and there was statistically significant difference between group B and group C (P=0.026). The PEACAM-1 expression was higher in group A than in groups B and C (20.31%±0.70% vs. 19.34%±0.68% vs. 18.37%±0.46%) (P<0.05), and there was statistically significant difference between group B and group C (P=0.017). Pearson's correlation analysis showed that the expression of PECAM-1 was negatively correlated to both ALI score (Coe=-0.732, P=0.001) and degree of LF (Coe=-0.779, P<0.001).

CONCLUSIONS: The PECAM-1 expression significantly decreases in New Zealand rabbits after PQ poisoning, and the decrease is dose-dependent. The PECAM-1 expression is negatively correlated with ALI score and LF, showing a significant role in the development of lung injuries induced by PQ.

Key words: Platelet endothelial cell adhesion molecule-1, Paraquat, Acute lung injury, Lung fibrosis

Jing Shi, Chun-lin Hu, Yu-feng Gao, Xiao-xing Liao, Hope Xu. The relationship between platelet endothelial cell adhesion molecule-1 and paraquat-induced lung injury in rabbits[J]. World Journal of Emergency Medicine, 2012, 3(1): 60-64.

Figures/Tables 5

Table 1

Table 2

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Groups	BW (kg)	HR (Times/min)	RR (Times/min)	T (°C)
A	2.41±0.26	236±36	32.3±2.34	38.5±2.34
B	2.45±0.23	245±56	29.8±4.56	38.2±1.78
C	2.38±0.46	239±27	30.4±5.26	39.1±1.05
P value	0.923	0.808	0.956	0.889

Groups	Survival time (day)
A	6.47±0.99
B	6.09±1.04
C	4.77±2.04^*#
P value	0.923

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