Effects of α-methylnorepinephrine on cardiac function and myocardium at early stage of resuscitation in rabbits

Abstract

Abstract:

BACKGROUND: Recent studies have shown that α2-adrenergic agonists can reduce postresuscitation myocardial injury. This study was undertaken to observe changes of hemodynamics, myocardial injury markers cTnT and cardiac morphology by establishing a cardiopulmonary resuscitation model with rabbits, and to detect whether α-methyl norepinephrine (α-MNE) can reduce the myocardial injury after CPR and improve cardiac function.

METHODS: Eighteen health rabbits, weighing 2.5-3.5 kg, both male and female, were provided by the Lanzhou Institute of Veterinary Medicine. After setting up a rabbit model of cardiopulmonary resuscitation, 18 rabbits were randomly divided into three groups. The rabbits in group A as an operation-control group were subjected to anesthesia, endotracheal intubation, and surgery without induction of ventricular fibrillation. The rabbits in group B as an epinephrine group were administered with 30 μg/kg epinephrineduring CPR. The rabbits in group C as a MNE group were administered with 100 μg/kg a-MNE during CPR. The left ventricular end-diastolic pressure (LVEDP), left ventricular pressure rise and fall rate (±dp/dt) and serum concentrations of BNP were measured. Statistical package of SPSS 10.0 was used for data analysis and significant differences between means were evaluated by ANOVA.

RESULTS: Compared to group A, the LVEDP of other two groups increased respectively (P<0.01 all), and peak±dp/dt decreased in the other two groups (P<0.01). The increase of LVEDP was lower in group C than in group B (P<0.05), whereas peak±dp/dt was higher in group C than in group B (P<0.05) at the same stage. Compared to group A, the cTnT of the remaining two groups increased, respectively (P<0.01), and peaked at 30 minutes. cTnT was less elevated in group C than in group B (P<0.05) during the same period. In groups B and C, myocardial injury was seen under a light microscope, but the injury in group C was lighter than that in group B.

CONCLUSION: Methylnorepinephrine can lessen myocardial dysfunction after CPR.

Key words: Cardiopulmonary resuscitation, α2-adrenergic agonist, Post-resuscitation myocardial dysfunction

Pei-jie Li, Xiao-hua Yang, Zheng-yi Zhang, Wen Cao, Li-ping Zhang, Jing Qin, Xiao-dong Wang. Effects of α-methylnorepinephrine on cardiac function and myocardium at early stage of resuscitation in rabbits[J]. World Journal of Emergency Medicine, 2010, 1(2): 132-137.

Figures/Tables 6

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Table 1

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Groups Before fibrilation		After restoration of spontaneous circulation
Groups Before fibrilation		30 min	60 min	120 min	180 min	240 min
A (n=5)	0.24±0.03	0.23±0.03	0.24±0.05	0.24±0.03	0.23±0.02	0.25±0.01
B (n=5)	0.25±0.07	0.44±0.09^*	0.52±0.12^*	0.55±0.14^*	0.55±0.14^*	0.59±0.15^*
C (n=6)	0.24±0.05	0.33±0.06^*#	0.37±0.06^*▲	0.41±0.09^*#	0.41±0.09^*#	0.43±0.94^*#

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