Mannitol-facilitated entry of vancomycin into the central nervous system inhibits neuroinflammation in a rat model of MRSA intracranial infection by modulating brain endothelial cells
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Mannitol-facilitated entry of vancomycin into the central nervous system inhibits neuroinflammation in a rat model of MRSA intracranial infection by modulating brain endothelial cells |
| Yin Wen, Zhiwei Su, Huishan Zhu, Mengting Liu, Zhuo Li, Shiying Zhang, Shuangming Cai, Jiaqi Tang, Hongguang Ding, Hongke Zeng |
| Figure 3. Mannitol reduced the EGL in vivo and in vitro. A, B: following mannitol administration, the expression levels of syndecan-1 in the brain tissue of rats sharply decreased at 5 min (P<0.05) and gradually returned to normal at 10, 30 and 60 min (P>0.05). C: the immunofluorescence signals of syndecan-1 (green) were faded at 5 min after mannitol administration, but were gradually restored at 10, 30 and 60 min; D, E: the expression levels of syndecan-1 of the cells decreased over time at 1, 3, 6, 12 and 24 h after mannitol intervention; F: the immunofluorescence signals of syndecan-1 (green) were faded in the Man group compared with the control group. *P<0.05, ***P<0.001, ****P<0.0001. EGL: endothelial glycocalyx layer. |
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