Mannitol-facilitated entry of vancomycin into the central nervous system inhibits neuroinflammation in a rat model of MRSA intracranial infection by modulating brain endothelial cells
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Mannitol-facilitated entry of vancomycin into the central nervous system inhibits neuroinflammation in a rat model of MRSA intracranial infection by modulating brain endothelial cells |
| Yin Wen, Zhiwei Su, Huishan Zhu, Mengting Liu, Zhuo Li, Shiying Zhang, Shuangming Cai, Jiaqi Tang, Hongguang Ding, Hongke Zeng |
| Figure 1. Mannitol promoted BBB opening for 30 min in rats and elevated the concentration of vancomycin in brain tissues. A: the Man group increased BBB permeability compared with the Con group (P<0.05); B: the concentration of NaF rapidly increased during initial 5-min period, and increased over 30 min following mannitol administration; the NaF concentration at 30 min showed no difference compared with that at 60 min; C: the concentration of FITC-dextran in the lower chamber of the Transwell was significantly higher in the Man group than that in the Con group (P<0.05); D: the concentration of vancomycin in the Man+Van group tended to increase at 5, 10, 30 and 60 min compared with that in the Van group (P<0.01). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. BBB: blood-brain barrier; NaF: sodium fluorescein; Man: mannitol; Van: vancomycin; FITC: fluorescein isothiocyanate. |
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