Hepatocellular carcinoma-derived exosomal miRNA-761 regulates the tumor microenvironment by targeting the SOCS2/JAK2/STAT3 pathway

Xiao-hu Zhou, Hao Xu, Chang Xu, Ying-cai Yan, Lin-shi Zhang, Qiang Sun, Wei-lin Wang, Yan-jun Shi

Figure 1. Characterization of exosomes from hepatocellular carcinoma (HCC) cells. A: the expression of exosomal miR-761 in five HCC cell lines and one healthy liver cell line determined by qRT-PCR; B: representative electron micrograph of exosomes isolated from Huh7 and LM3 cells (scale bar: 100 nm); C: size of exosomes in Huh7 and LM3 cells; D: detection of exosomal miR-761 secreted by Huh7 cells in northern blotting, U6 as an internal control; E: exosomes isolated from Huh7 and LM3 cells, expression of CD81 and TSG101 examined by Western blotting using specific antibodies. qRT-PCR: quantitative reverse transcription polymerase chain reaction; *P<0.01; **P<0.001. The black arrow: exosome.