Trichostatin A improves the inflammatory response and liver injury in septic mice through the FoxO3a/autophagy signaling pathway

Mei-jia Shen, Li-chao Sun, Xiao-yu Liu, Meng-chen Xiong, Shan Li, A-ling Tang, Guo-qiang Zhang

Figure 2. FoxO3a was involved in the induction of autophagy and the improvement of liver injury and inflammation in the sepsis cell model by TSA. The expression of IL-6 (A) and TNF-α (B) measured by ELISA; the expression of LC3, P62, and FoxO3a analyzed by Western blotting (C); immunofluorescence results of LC3 (D) and FoxO3a (E) detected by confocal microscopy (400×); the changes of LC3 and P62 expression after FoxO3a gene knocked down in AML12 cells in each group analyzed by Western blotting (F). FoxO3a: forkhead box O3a; IL-6: interleukin-6; TNF-α: tumor necrosis factor-α; TSA: trichostatin A; LPS: lipopolysaccharide; ELISA: enzyme-linked immunosorbent assay; DAPI: 4',6-diamidino-2-phenylindole. Compared with control group, *P<0.05, **P<0.01.