Trichostatin A improves the inflammatory response and liver injury in septic mice through the FoxO3a/autophagy signaling pathway

Mei-jia Shen, Li-chao Sun, Xiao-yu Liu, Meng-chen Xiong, Shan Li, A-ling Tang, Guo-qiang Zhang

Table 3. Comparison of ALT, AST, IL-6, TNF-α, LC3 II, and P62 after FoxO3a gene knocked down in AML12 cells among groups

Parameters	Control group	si-negative control			si-FoxO3a
Parameters	Control group	TSA group	LPS group	LPS+TSA group	TSA group	LPS group	LPS+TSA group
ALT ^a, U/L	5.37±0.63	14.13±1.80	29.27±0.52	23.33±0.50	14.63±1.03	38.57±2.30^**	29.20±1.10^**
AST ^a, U/L	9.07±0.87	20.27±3.18	57.00±1.59	39.57±1.94	19.63±3.03	71.23±5.85^*	46.70±2.90^*
IL-6 ^a, pg/mL	40.05±1.96	111.77±2.53	500.50±7.96	355.07±9.71	132.07±2.76^**	598.57±14.01^**	413.73±26.24^*
TNF-α ^a, pg/mL	10.33±1.25	11.93±1.69	54.15±2.79	36.77±2.39	13.47±1.92^*	66.47±3.03^*	50.43±3.35^**
LC3 II^b	1	1.51±0.01	2.51±0.02	2.57±0.01	1.22±0.01^**	1.69±0.01^**	1.28±0.04^**
P62 ^b	1	0.48±0.01	0.54±0.01	0.33±0.01	0.94±0.01^**	0.66±0.01^**	0.93±0.02^**