Role of epithelial-to-mesenchymal transition in the pulmonary fibrosis induced by paraquat in rats

Jian-hua Yi, Zhao-cai Zhang, Mei-bian Zhang, Xin He, Hao-ran Lin, Hai-wen Huang, Hai-bin Dai, Yu-wen Huang

Table 1 Sequences of the primers used in real-time RT-PCR

Target gene Primer sequence Length of the product (bp) E-cadherin F: GGGTTGTCTCAGCCAATGTT R: CACCAACACACCCAGCATAG 206 α-SMA F: AGCCAGTCGCCATCAGGAAC R: CCGGAGCCATTGTCACACAC 242 FSP-1 F: AGGCAACGAGGGTGACAAGTTC R: CATCATGGCAATGCAGGACAG 194 Slug (snail2) F: GCACTGTGATGCCCAGGCTA R: CCTTGCCACAGATCTTGCAGAC 215 Twist F: ACCCTCACACCTCTGCATTC R: CAGTTTGATCCCAGCGTTTT 228 TGF-β1 F: ATACGCCTGAGTGGCTGTCT R: TGGGACTGATCCCATTGATT 225 Smad2 F: CCAGGTCTCTTGATGGTCGT R: TCTCCACCCTCTGGTAGTGG 241 Smad7 F: 5'TCCTGCTGTGCAAAGTGTTC3' R: 5'TCTGGACAGTCTGCAGTTGG3' 188 Wnt2 F: GTGTGACAATGTGCCAGGTC R: GTGGTCTCTGTCCAGGGTGT 182 β-catenin F: GCCAGTGGATTCCGTACTGT R: GAGCTTGCTTTCCTGATTGC 183 GAPDH F: AGACAGCCGCATCTTCTTGT R: CTTGCCGTGGGTAGAGTCAT 207