World Journal of Emergency Medicine ›› 2013, Vol. 4 ›› Issue (1): 32-37.doi: 10.5847/wjem.j.issn.1920-8642.2013.01.006
• Original Articles • Previous Articles Next Articles
Gan-nan Wang1, Jin-song Zhang1(), Wei-juan Cao1, Hao Sun1, Jing Zhang1, Yao Wang1, Hang Xiao2
Received:
2012-08-16
Accepted:
2012-12-03
Online:
2013-03-15
Published:
2013-03-15
Contact:
Jin-song Zhang
E-mail:zhangjso@sina.com
Gan-nan Wang, Jin-song Zhang, Wei-juan Cao, Hao Sun, Jing Zhang, Yao Wang, Hang Xiao. Association of ALOX5, LTA4H and LTC4S gene polymorphisms with ischemic stroke risk in a cohort of Chinese in east China[J]. World Journal of Emergency Medicine, 2013, 4(1): 32-37.
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URL: http://wjem.com.cn//EN/10.5847/wjem.j.issn.1920-8642.2013.01.006
Table 2
Primers and restriction enzymes for PCR-RFLP genotyping
SNPs | Sequence of primers (5'-3')* | Tanneal (°C) | Length of PCR (bp) | Restriction enzymes | Length of fragment (bp) |
---|---|---|---|---|---|
rs2029253 A>G | F: TTCCACAGTGTATGGCCTGG(T→G)C R: ACTCTCCTCTCCATCTCTTGTCTGGA | 60.0 | 120 | Hin6I | AA: 120 |
AG: 120, 100, 20 | |||||
GG: 100, 20 | |||||
rs6538697 T>C | F: TCTCCGTAAATCATGCTTGCTG(T→G)GTAC R: ACGGAGTCCCAGTCACAACTCT | 59.4 | 144 | Acc65I | TT: 144 |
TC: 144, 122, 22 | |||||
CC: 122, 22 | |||||
rs730012 A>C | F: CAGGAACAGCCTGGATGGGT(G→T)AC R: ACTTTCTCCAGGGCCTTGCAG | 62.8 | 112 | KpnI | AA: 112 |
AC: 112, 90, 22 | |||||
CC: 90, 22 |
Table 3
Clinical characteristics of the controls and IS patients
Variables | Controls (n=690) | Cases (n=690) | P |
---|---|---|---|
Age (y) | 67.01±9.59 | 67.87±9.52 | 0.097 |
Male (%) | 54.6 | 59.7 | 0.057 |
BMI (kg/m2) | 23.32±2.39 | 24.20±3.21 | <0.001 |
Smoking (%) | 22.8 | 30.3 | 0.002 |
Hypertension (%) | 26.8 | 77.1 | <0.001 |
Diabetes (%) | 11.6 | 34.1 | <0.001 |
SBP (mmHg) | 124.98±17.47 | 144.09±22.17 | <0.001 |
DBP (mmHg) | 79.38±31.58 | 83.69±11.80 | 0.001 |
TC (mmol/L) | 4.48±1.22 | 4.67±1.17 | 0.003 |
TG (mmol/L) | 1.38±0.98 | 1.67±1.19 | <0.001 |
HDL-C (mmol/L) | 1.28±0.36 | 1.15±0.34 | <0.001 |
LDL-C (mmol/L) | 2.55±0.76 | 2.79±0.84 | <0.001 |
Table 4
Genotypic distributions and allelic frequencies of SNPs
Gene | SNPs | Alleles*(1/2) | Group | Genotypes (n, %) | P▲ | 2 vs. 1 | |||
---|---|---|---|---|---|---|---|---|---|
1/1 | 1/2 | 2/2 | P | OR (95%CI) | |||||
ALOX5 | rs2029253 | G/A | Control | 274 (39.7) | 318 (46.1) | 98 (14.2) | — | 1.00 (Ref.) | |
Patient | 224 (32.5) | 388 (56.2) | 78 (11.3) | 0.001 | 0.240 | 1.10 (0.94-1.28) | |||
LTA4H | rs6538697 | T/C | Control | 376 (54.5) | 275 (39.9) | 39 (5.7) | — | — | 1.00 (Ref.) |
Patient | 362 (52.5) | 267 (38.7) | 61 (8.8) | 0.073 | 0.122 | 1.14 (0.97-1.35) | |||
LTC4S | rs730012 | A/C | Control | 556 (80.6) | 131 (19.0) | 3 (0.4) | — | — | 1.00 (Ref.) |
Patient | 522 (75.7) | 155 (22.5) | 13 (1.9) | 0.009 | 0.009 | 1.37 (1.08-1.73) |
Table 5
Association results of SNPs with ischemic stroke risk according to the recessive genetic model
SNPs | Genotype | Controls | Patients | Unadjusted | Adjusted | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
n | % | n | % | OR (95%CI) | P | OR (95%CI) | P | |||||
ALOX5 | ||||||||||||
rs2029253 | AA, AG | 416 | 60.3 | 466 | 67.5 | 1.00 (Ref.) | — | 1.00 (Ref.) | — | |||
GG | 274 | 39.7 | 224 | 32.5 | 0.73 (0.59-0.91) | 0.005 | 0.72 (0.55-0.93) | 0.013 | ||||
LTA4H | ||||||||||||
rs6538697 | TT, TC | 651 | 94.3 | 629 | 91.2 | 1.00 (Ref.) | — | 1.00 (Ref.) | — | |||
CC | 39 | 5.7 | 61 | 8.8 | 1.62 (1.07-2.46) | 0.022 | 1.77 (1.09-2.89) | 0.022 | ||||
LTC4S | ||||||||||||
rs730012 | AA, AC | 687 | 99.6 | 677 | 98.1 | 1.00 (Ref.) | — | 1.00 (Ref.) | — | |||
CC | 3 | 0.4 | 13 | 1.9 | 4.40 (1.25-15.50) | 0.012 | 3.77 (0.92-15.50) | 0.066 |
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