Intravenous injection of Xuebijing attenuates acute kidney injury in rats with paraquat intoxication

doi:10.5847/wjem.j.1920-8642.2017.01.011

Abstract

Abstract:

BACKGROUND: The study aimed to investigate the therapeutic benefits of intravenous Xuebijing on acute kidney injury (AKI) in rats with paraquat intoxication.

METHODS: Male Sprague-Dawley rats were randomly divided equally into three groups: sham group (n=8), paraquat group (n=8) and Xuebijing-treated group (n=8) using a random number table. The rats were intraperitoneally injected with 50 mg/kg of paraquat. One hour after paraquat administration, the rats were treated intravenously with Xuebijing (8 mL/kg). At 12 hours after paraquat administration, serum was collected to evaluate kidney function, then the rats were sacrificed and kidney samples were immediately harvested. AKI scores were evaluated by renal histopathology and pro-inflammatory cytokines mRNA levels in kidney were assayed using real-time RT-PCR.

RESULTS: Serum urea nitrogen, creatinine and AKI scores were significantly higher in the paraquat group, compared with the sham group (P<0.05, respectively). Moreover, interleukin (IL)-1β, IL-6 and TNF-α mRNA levels were significantly higher in the paraquat group (P<0.01, respectively). However, intravenous Xuebijing significantly decreased serum urea nitrogen, creatinine, AKI scores and IL-1β, IL-6 and TNF-α mRNA levels, compared with the paraquat group (P<0.05, respectively).

CONCLUSION: Intravenous Xuebijing attenuates AKI following paraquat poisoning by suppressing inflammatory response.

Key words: Paraquat, Xuebijing, Kidney injury, Inflammation

Jia-jun Xu, Jian-tao Zhen, Li Tang, Qing-ming Lin. Intravenous injection of Xuebijing attenuates acute kidney injury in rats with paraquat intoxication[J]. World Journal of Emergency Medicine, 2017, 8(1): 61-64.

Figures/Tables 3

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Groups	n	BUN (mmol/L)	Cr (μmol/L)	AKI scores
Sham	8	6.11±0.53	58.42±5.67	0.26±0.09
Paraquat	8	20.81±3.14^*	165.75±16.29^*	8.57±0.42^*
Paraquat Xuebijing-treated	8	15.28±4.36^*#	129.16±10.44^*#	5.64±0.36^*#

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