Role of epithelial-to-mesenchymal transition in the pulmonary fibrosis induced by paraquat in rats

doi:10.5847/wjem.j.1920-8642.2021.03.009

世界急诊医学杂志（英文） ›› 2021, Vol. 12 ›› Issue (3): 214-220.doi: 10.5847/wjem.j.1920-8642.2021.03.009

收稿日期:2020-08-10 接受日期:2021-02-21 出版日期:2021-06-01 发布日期:2021-05-31

Role of epithelial-to-mesenchymal transition in the pulmonary fibrosis induced by paraquat in rats

Jian-hua Yi¹, Zhao-cai Zhang², Mei-bian Zhang³, Xin He⁴, Hao-ran Lin⁵, Hai-wen Huang², Hai-bin Dai⁵, Yu-wen Huang⁵()

¹ Emergency Department, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China
² Scientific Research Department, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China
³ Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 301051, China
⁴ Shangyu People’s Hospital, Shaoxing 312300, China
⁵ Pharmacy Department, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China

Received:2020-08-10 Accepted:2021-02-21 Online:2021-06-01 Published:2021-05-31
Contact: Yu-wen Huang E-mail:2504152@zju.edu.cn

摘要/Abstract

Abstract:

BACKGROUND: This study aims to explore the characteristics of the epithelial-to-mesenchymal transition (EMT) process and its underlying molecular mechanisms in the period of paraquat (PQ)-induced pulmonary fibrosis (PF).

METHODS: Picrosirius red staining and collagen volume fraction were utilized to evaluate the pathological changes of PQ-induced PF in rats. Immunohistochemistry, Western blot, and real-time reverse transcriptase-polymerase chain reaction (RT-PCR) were used to measure the protein and gene expression of EMT markers, EMT-associated transcription factors, and regulators of EMT-related pathways, respectively.

RESULTS: The collagen deposition in the alveolar septum and increased PF markers were characteristics of pathological changes in PQ-induced PF, reached a peak on day 14 after PQ poisoning, and then decreased on day 21. The protein and gene expression of the fibrosis marker, EMT markers, transcription factors, and regulators of EMT-related signaling pathways significantly increased at different time points after PQ poisoning compared with corresponding controls (P<0.05), and most of them reached a peak on day 14, followed by a decrease on day 21. The gene expression of EMT markers was significantly correlated with PF markers, transcription factors, and regulators of EMT-related signaling pathways (P<0.05). The mRNA expression of transcription factors was significantly correlated with that of TGF-β1 and Smad2 (P<0.05 or P<0.01), instead of Wnt2 and β-catenin (P>0.05).

CONCLUSIONS: EMT process plays a role in the PQ-induced PF, in which most PF and EMT markers have a peak phenomenon, and its underlying molecular mechanisms might be determined by further studies.

Key words: Pulmonary fibrosis, Paraquat, Epithelial-to-mesenchymal transition, Rats

. [J]. 世界急诊医学杂志（英文）, 2021, 12(3): 214-220.

Jian-hua Yi, Zhao-cai Zhang, Mei-bian Zhang, Xin He, Hao-ran Lin, Hai-wen Huang, Hai-bin Dai, Yu-wen Huang. Role of epithelial-to-mesenchymal transition in the pulmonary fibrosis induced by paraquat in rats[J]. World Journal of Emergency Medicine, 2021, 12(3): 214-220.

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参考文献 30

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Target gene	Primer sequence	Length of the product (bp)
E-cadherin	F: GGGTTGTCTCAGCCAATGTT R: CACCAACACACCCAGCATAG	206
α-SMA	F: AGCCAGTCGCCATCAGGAAC R: CCGGAGCCATTGTCACACAC	242
FSP-1	F: AGGCAACGAGGGTGACAAGTTC R: CATCATGGCAATGCAGGACAG	194
Slug (snail2)	F: GCACTGTGATGCCCAGGCTA R: CCTTGCCACAGATCTTGCAGAC	215
Twist	F: ACCCTCACACCTCTGCATTC R: CAGTTTGATCCCAGCGTTTT	228
TGF-β1	F: ATACGCCTGAGTGGCTGTCT R: TGGGACTGATCCCATTGATT	225
Smad2	F: CCAGGTCTCTTGATGGTCGT R: TCTCCACCCTCTGGTAGTGG	241
Smad7	F: 5'TCCTGCTGTGCAAAGTGTTC3' R: 5'TCTGGACAGTCTGCAGTTGG3'	188
Wnt2	F: GTGTGACAATGTGCCAGGTC R: GTGGTCTCTGTCCAGGGTGT	182
β-catenin	F: GCCAGTGGATTCCGTACTGT R: GAGCTTGCTTTCCTGATTGC	183
GAPDH	F: AGACAGCCGCATCTTCTTGT R: CTTGCCGTGGGTAGAGTCAT	207

Parameters	α-SMA	E-cadherin	Slug	Twist	TGF-β1	Smad2	Wnt2	β-catenin
α-SMA	1	-	-	-	-	-	-	-
E-cadherin	-0.818^*	1	-	-	-	-	-	-
Slug	0.869^*	-0.752^*	1	-	-	-	-	-
Twist	0.922^*	-0.845^*	0.960^#	1	-	-	-	-
TGF-β1	0.955^#	-0.909^#	0.822^*	0.863^*	1	-	-	-
Smad2	0.894^*	-0.818^*	0.971^#	0.993^#	0.971^*	1	-	-
Wnt2	0.869^*	-0.942^#	0.521	0.646	0.800	0.601	1	-
β-catenin	0.797	-0.853^*	0.447	0.579	0.679	0.533	0.959^#	1

Role of epithelial-to-mesenchymal transition in the pulmonary fibrosis induced by paraquat in rats

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